Southern Ocean Ecosystem cruise 2019 biogeochemistry
Phytoplankton, pigment, bacteria and biogeochemistry data from an ecosystem cruise to Kong Håkon VII Hav, Southern Ocean, in March 2019 with the R/V Kronprins Haakon.
Simple
- Date (Revision)
- 2021-08-30
- Credit
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Sebastien Moreau
- Credit
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Hanna Kauko
- Credit
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Thomas Ryan-Keogh
- Credit
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Asmita Singh
- Credit
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Ilka Peeken
- Credit
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Gunnar Bratbak
- Status
- On going
- Theme
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oceanography
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marine
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biology
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ecology
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- Global Change Master Directory (GCMD) Keywords
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OCEANS | OCEAN CHEMISTRY | PIGMENTS | CHLOROPHYLL
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BIOSPHERE | AQUATIC ECOSYSTEMS | PLANKTON | PHYTOPLANKTON
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OCEANS | SALINITY/DENSITY
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BIOSPHERE | ECOLOGICAL DYNAMICS
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- Topic category
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- Biota
- Oceans
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- Begin date
- 2019-02-26
- End date
- 2019-04-11
- Distribution format
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JSON (edit URI) (application/json)
()
Distributor
- OnLine resource
- https://api.npolar.no/dataset/28fbddd2-0fb2-41c9-9f42-60146e28617f ( WWW:DOWNLOAD-1.0-http--download )
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Data (application/zip)
()
Distributor
- OnLine resource
- https://api.npolar.no/dataset/28fbddd2-0fb2-41c9-9f42-60146e28617f/_file/_all/?filename=&format=zip ( WWW:DOWNLOAD-1.0-http--download )
-
JSON (edit URI) (application/json)
()
- OnLine resource
- https://api.npolar.no/dataset/28fbddd2-0fb2-41c9-9f42-60146e28617f ( WWW:DOWNLOAD-1.0-http--download )
- Statement
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Chlorophyll (Chl) a samples were collected from multiple depths, filtered (500-1000 ml, typically 1000 ml) with a gentle vacuum pressure on 0.7 um GF/F filters, and extracted in 100 % methanol in darkness, 5° C and for 24 h. Pigment concentration was measured with a calibrated Turner 10-AU Fluorometer (Turner Designs, San Jose, USA).
Flow cytometry samples (FCM) were filled in 4.5 ml cryovials, fixed with glutaraldehyde (final concentration 0.5 %), and stored in -80° C until analysis. The samples were analysed with a Attune™ NxT Acoustic Focusing Cytometer (Invitrogen™, Thermo Fisher Scientific Inc. USA) at the University of Bergen, Norway 6 months after the campaign (September 2019). Before the analysis, the bacteria samples were diluted (10x) with filtered TE buffer, stained with SYBR Green I and incubated at room temperature in darkness for 10 minutes. Bacteria concentration estimates were based on side scatter and green fluorescence. Pico- and nanophytoplankton concentration estimates (included in version 2) were based on side scatter and red fluorescence.
[To be included in version 2 in 2022:]
Samples for HPLC analysis were collected in the same way and stored in -80° C until analysis at the Alfred Wegener Institute (Bremen, Germany) with a Waters Alliance 2695 HPLC Separation Module, Waters photodiode array detector (2,996), Agilent Technologies Microsorb-MV3 C8 column (4.6 × 100 mm), and 1 molar ammonium acetate solution. For more details see the below mentioned articles. Header explanations: Chla - chlorophyll a; Chlb - chlorophyll b; Chlc1_Chlc2 - chlorophyll c1 + chlorophyll c2; Chlc3 - chlorophyll c3; Fucox - fucoxanthin; 19But - 19’butanoyloxyfucoxanthin; 19Hex - 19’hexanoyloxyfucoxanthin; Perid - peridinin; Lutein - lutein; Allox – alloxanthin; Zeax - zeaxanthin; DD - diadinoxanthin; DT – diatoxanthin; Beta_car - beta carotene.
Also included here Chl a concentrations from an underway fluorometer (WETStar, Sea-Bird Scientific; scientific seawater intake at 4 m depth) calibrated with Chl a samples (taken every 4 hours during longer transits; y=0.09x + 0.06; R2=0.85) processed with the above mentioned method.
Unpublished data to be included in future versions (v3): biogenic silica, particulate organic carbon and nitrogen (POC and PON), dissolved organic carbon (DOC) and total dissolved nitrogen (TDN).
For more details see [Kauko et al. (2021)]( https://doi.org/10.3389/fmars.2021.623856) and Kauko et al. (in prep). See also [Chl a vertical profiles]( https://data.npolar.no/dataset/5e510f85-29e1-42c3-89a5-8766f5c5948c) from an in situ fluorometer, [taxonomy and abundance data]( https://data.npolar.no/dataset/283e500c-732b-4f9b-a48a-3bc4990e3f55) and [nutrients data]( https://doi.org/10.21335/NMDC-1503664923). The microscopy sample IDs (taxonomy and abundance) are included in this dataset (v2) for easier combination.
Regarding the underway data, see also [Ogundare et al. (2021)]( https://doi.org/10.3389/fmars.2020.614263) and [this dataset]( https://doi.org/10.21334/npolar.2020.cc4bfb5f).
- File identifier
- 28fbddd2-0fb2-41c9-9f42-60146e28617f XML
- Metadata language
- English
- Hierarchy level
- Dataset
- Date stamp
- 2022-05-27
- Metadata standard name
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CEOS IDN DIF
- Metadata standard version
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9.8.4
Overviews
Spatial extent
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