geonetworktest
Concentrations of phthtalates and their potential to transactivate nuclear receptors in marine mammals from the Norwegian Arctic
Phthalate diester concentrations were measured in blubber/adipose tissue of blue whales (Balaenoptera musculus), fin whales (Balaenoptera physalus), bowhead whales (Balaena mysticetus) and polar bears (Ursus maritimus) sampled from the Barents and Greenland seas. Additionally, plasma samples from polar bears were analysed for total concentrations of phthalate monoester metabolites including their free and glucuronated forms. In vitro reporter gene assays were used to assess transcriptional activity of fin whale nuclear receptors by selected phthalates. Membrane integrity and metabolic activity were monitored as a measure for the viability of the cells used for reportor gene assays.
Simple
- Date (Revision)
- 2021-03-01
- Credit
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Heli Routti
- Credit
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Mikael Harju
- Credit
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Katharina Luhmann
- Credit
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Jon Aars
- Credit
-
Anders Goksøyr
- Credit
-
Kit M. Kovacs
- Credit
-
Christian Lydersen
- Status
- Completed
- Theme
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Svalbard, Fram Strait
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ecotoxicology
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marine
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- Global Change Master Directory (GCMD) Keywords
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BIOLOGICAL CLASSIFICATION | ANIMALS/VERTEBRATES | MAMMALS | CETACEANS | BALEEN WHALES
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BIOLOGICAL CLASSIFICATION | ANIMALS/VERTEBRATES | MAMMALS | CARNIVORES
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BIOSPHERE | ECOLOGICAL DYNAMICS | ECOTOXICOLOGY
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- Topic category
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- Biota
- Environment
- Health
- Oceans
- Begin date
- 2017-03-23
- End date
- 2019-06-02
- Distribution format
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JSON (edit URI) (application/json)
()
Distributor
- OnLine resource
- https://api.npolar.no/dataset/5499f1ad-cbca-4aa5-b986-5891f0c60ad2 ( WWW:DOWNLOAD-1.0-http--download )
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Data (application/zip)
()
Distributor
- OnLine resource
- https://api.npolar.no/dataset/5499f1ad-cbca-4aa5-b986-5891f0c60ad2/_file/_all/?filename=&format=zip ( WWW:DOWNLOAD-1.0-http--download )
-
JSON (edit URI) (application/json)
()
- OnLine resource
- https://api.npolar.no/dataset/5499f1ad-cbca-4aa5-b986-5891f0c60ad2 ( WWW:DOWNLOAD-1.0-http--download )
- Statement
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The agonistic and antagonistic effects of bis(2-ethylhexyl) phthalate (DEHP) and diisononyl phthalate (DiNP) on the receptor plasmids encoding for fin whale ligand binding domains of peroxisome proliferator-activated receptor gamma (PPARG), glucocorticoid receptor (GR) and the thyroid hormone receptor beta (THRB) were tested by luciferase gene reporter assays. COS7 cells were transiently transfected with the reporter plasmid tk(MH100)x4-luciferase, control plasmid pCMV-β-galactosidase, and either of the receptor plasmids pCMX-Gal4-wPPARG, pCMX-Gal4-wGR or pCMX-Gal4-wTHRB coding for fusion proteins of the yeast GAL4 DNA binding domain and the LBD of the corresponding receptor. The cells were exposed to test compounds and known ligands dissolved in a solvent for 24 h. Antagonistic effects of test compounds were tested in the presence of a known ligand. Luciferase and β-galactosidase activities were assayed in cell lysates as changes in luminescence (response) and absorbance (beta.gal), respectively.
The dye resazurin was used to test for metabolic activity and 5-carboxyfluorescein diacetate (CFDA-AM) was used for to determine membrane integrity when exposed to DEHP and DiNP. The positive control was Triton X-100, which is known to be cytotoxic for mammalian cells. The response is a measure of fluoresence.
- File identifier
- 5499f1ad-cbca-4aa5-b986-5891f0c60ad2 XML
- Metadata language
- English
- Hierarchy level
- Dataset
- Date stamp
- 2022-05-27
- Metadata standard name
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CEOS IDN DIF
- Metadata standard version
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9.8.4
